Hey everyone! I’ve been struggling lately with RNA extraction from some really fibrous tissue samples – mainly cardiac and skeletal muscle. No matter how careful I am, I keep ending up with degraded RNA or extremely low yields. I’ve tried Trizol and even switched to a column-based kit, but the quality still isn’t great. Anyone had better luck with tougher tissues like this? Would really appreciate any tips or methods that worked for you!
Hey everyone! I’ve been struggling lately with RNA extraction from some really fibrous tissue samples – mainly cardiac and skeletal muscle. No matter how careful I am, I keep ending up with degraded RNA or extremely low yields. I’ve tried Trizol what is chromatography and even switched to a column-based kit, but the quality still isn’t great. Anyone had better luck with tougher tissues like this? Would really appreciate any tips or methods that worked for you!
I’ve been struggling lately with RNA extraction from some really fibrous tissue samples – mainly cardiac and skeletal muscle. No matter how careful I am, I keep ending up with degraded RNA or extremely low yields. I’ve tried Trizol and even switched to a column-based kit, but the quality still isn’t great.
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